ABSTRACT

Immunofluorescence and immunohistochemistry are two methods with similar procedures that are commonly used to evaluate expression of proteins in tissues. Their value is that expression can be identified in specific cells, organs, and structures, as opposed to immunoblots, which merely indicate whether expression is present or not in an organ. Immunofluorescence and immunohistochemistry are qualitative, while immunoblots can be quantitative or semiquantitative, so each does provide advantages.