ABSTRACT
Introduction..............................................................................................................23
E. coli
Expression....................................................................................................24 Introduction..................................................................................................24 Optimizing Soluble Protein Expression ......................................................24 HTP Methods for Detecting Protein Solubility ..........................................26 Protein Purification ......................................................................................27
Yeast Expression......................................................................................................27 Introduction..................................................................................................27 Early Heterologous Protein Expression in Yeast ........................................28 Pichia pastoris Expression Systems ............................................................29 Posttranslational Modifications ...................................................................29 HTP Yeast Expression .................................................................................30
Insect Cell Expression .............................................................................................31 Introduction..................................................................................................31 Cloning for Insect Cell Expression .............................................................31 Posttranslational Modifications ...................................................................32 HTP Baculovirus Expression ......................................................................33
Choosing Expression Technologies for a HTP Pipeline.........................................34 References................................................................................................................35
