ABSTRACT
Introduction............................................................................................................146 Genetic Study of Multiprotein Complexes in
Saccharomyces cerevisiae
............146 Multiprotein Complexes ............................................................................146 Gene Disruption in Yeast...........................................................................149 Tagging Proteins in Yeast ..........................................................................150 Measurement of Global Transcription in Yeast .......................................152 Studies of the Med2 Subunit of the Yeast Mediator Complex.................153
Genetics to Study Single Proteins in Transgenic Mice ........................................155 Designing Transgenes................................................................................155 Modification of Transgenes .......................................................................155 Preparation of DNA for Micro-injection into the Pronucleus..................157 Micro-injection of DNA into the Zygote..................................................158 Analysis of Transgenic Animals................................................................159 Analysis of the
