ABSTRACT
Mature testes usually have a milky appearance and are located above the gonopodial area. ƒ Use the tweezers to grasp the posterior tubular portion of the testes and remove
them from the body cavity while taking care to avoid sperm release. ƒ Place the testes in resealable plastic bags, weigh, add sufficient HBSS based on
a 1:10 ratio of testis weight to HBSS volume, seal the bag, and crush the testis with the plastic goblet to release sperm. ƒ Transfer the sperm solution from the plastic bag to 1.5 ml centrifuge tubes, and
place it on crushed ice prior to use.
