ABSTRACT
Introduction.............................................................................................................. 20 Monitoring of Receptor Mediated cAMP Changes in Living Cells by Electrophysiological Approach................................................................................ 21
Experimental System, Setup, and Data Analysis........................................ 21 Examples...................................................................................................... 25 Conclusion ................................................................................................... 25
Förster Resonance Energy Transfer (FRET)-Based cAMP Sensor........................ 27 cAMP Sensors ............................................................................................. 27 FRET Principles and FRET-Based Analysis............................................... 28 Experimental System for cAMP Analysis by Using Epac1 Sensor........... 31
Light Sources ..................................................................................... 31 Optics ................................................................................................. 31 Camera ............................................................................................... 33
Examples...................................................................................................... 33 Outlook ........................................................................................................ 35
Acknowledgments.................................................................................................... 36 References................................................................................................................ 37
Five-hydroxytryptamine (5-HT or serotonin) is an important neuromodulator involved in a wide range of physiological functions. The effects of serotonin are mediated by a large family of receptors, either ionotropic or coupled to secondmessenger cascades. With the exception of the 5-HT
receptor, which is a cation channel, all 5-HT receptors belong to the superfamily of 7 transmembrane-spanning receptors that are coupled to multiple heterotrimeric G-proteins.
