ABSTRACT
Introduction .................................................................................................................................. 465
NO Microelectrodes ..................................................................................................................... 466
Clark Type NO Microelectrodes .............................................................................................. 467
Metalloporphyrin and Metallophthalocyanine Modified NO Microelectrodes ....................... 468
Combination NO Microelectrodes ........................................................................................... 469
Other NO Microelectrodes ....................................................................................................... 470
Calibration of NO Microelectrodes ............................................................................................. 470
Calibration Based on Chemical Generation of NO ................................................................. 470
Calibration Using an NO Standard Solution ........................................................................... 471
Calibration Based on Decomposition of SNAP ...................................................................... 471
Characterization of NO Microelectrodes ..................................................................................... 473
Sensitivity and Detection Limit ............................................................................................... 473
Selectivity ................................................................................................................................. 473
Response Time ......................................................................................................................... 474
Selected NO Microelectrode Applications .................................................................................. 474
Concluding Remarks .................................................................................................................... 481
Acknowledgments ........................................................................................................................ 481
References .................................................................................................................................... 482
Nitric oxide (NO) is a hydrophobic, highly labile free radical that is catalytically produced in
biological systems from the reduction of L-arginine by nitric oxide synthase (NOS) to form
L-citrulline, which produces NO in the process. In biological systems NO has long been known
to play various roles in physiology, pathology and pharmacology.
