ABSTRACT
Introduction .................................................................................................................................. 49
Advantages of the Use of Mouse Brain Slices ............................................................................ 50
Methodology for Fast Scan Cyclic Voltammetry in Mouse Brain Slices .................................. 51
Electrode Fabrication and Calibration ..................................................................................... 51
FSCV Data Acquisition............................................................................................................ 52
Brain Slice Preparation ............................................................................................................ 53
Electrically Stimulated Monoamine Release ........................................................................... 54
Electrical Stimulation ........................................................................................................... 54
Advantages of the Use of Electrical Stimulation ................................................................ 55
Experimental Design ................................................................................................................ 55
Data Analysis............................................................................................................................ 55
Uptake Rate Determination .................................................................................................. 55
Regional Variation in Uptake Rates .................................................................................... 57
Monoamine Uptake Inhibitors and Releasers .............................................................................. 58
Mechanisms of Action ............................................................................................................. 58
Using FSCV to Evaluate Changes in Monoamine Dynamics in Response
to Uptake Inhibitors and Releasers .......................................................................................... 58
Concluding remarks ..................................................................................................................... 60
Acknowledgments ........................................................................................................................ 60
References .................................................................................................................................... 60
Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that can be used to monitor
release and uptake dynamics of endogenous monoamine levels both in vitro
and in vivo.
