ABSTRACT
The coupling of high-performance liquid chromatography (HPLC) with nuclear magnetic resonance spectroscopy (LC-NMR) is one of the most powerful methods for the separation and structural elucidation of unknown compounds in mixtures. Recent progress in pulse eld gradients and solvent suppression, improvement in probe technology, and construction of high-eld magnets have given a new impulse to this technique, which has emerged since the mid-1990s as a very efcient method for the online identication of organic molecules. It took nearly 20 years to establish LC-NMR, and this long period can be mainly attributed to the intrinsically low sensitivity of NMR.
