ABSTRACT
The most important current problem of planar chromatography is the elaboration of theoretical and experimental methods for predicting the conditions of mixture separation in order to achieve better results. Planar chromatography is an analytical chemistry technique for the separation of mixtures that involves passing of solutes in the mobile phase through the stationary phase. Usually, each component has a
characteristic separation rate that can be used to identify it and the composition of the original mixture as well. Analytical thin-layer chromatography (TLC) is used to determine the compounds that are in a mixture and their concentrations. Preparative layer chromatography (PLC) may be defined as the TLC of relatively large amounts of material used in order to prepare and isolate quantities of separated compounds for further investigations such as chemical derivatization, structure elucidation (MS, NMR, IR, UV, etc.), and chromatographic standards or biological activity determination [1-4]. PLC is an excellent method for cleaning up synthetic reaction mixtures, natural products, plant extracts, and biotechnological products.
