ABSTRACT

Agar ..................................................................20.0g Peptone..............................................................10.0g Casamino acids ...................................................5.0g Malt extract .........................................................5.0g Yeast extract........................................................5.0g Beef extract .........................................................2.0g Glycerol ..............................................................2.0g MgSO4·7H2O ......................................................1.0g Tween™ 80....................................................50.0mg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121°C. Pour into sterile Petri dishes or leave in tubes.