ABSTRACT
Base Agar: Composition per liter: Papaic digest of soybean meal ..........................20.0g Agarose .............................................................10.0g NaCl ....................................................................5.0g Phenol Red (2% solution) ............................... 1.0mL
Preparation of Base Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure-121°C. Cool to 45°–50°C.
