ABSTRACT
Composition per liter: Agar .................................................................15.0g Casamino acids ..................................................1.0g Tris buffer ..........................................................1.0g MgSO4·7H2O .....................................................0.1g KNO3 .................................................................0.1g CaCl2·2H2O........................................................0.1g Sodium-β-glycerophosphate ..............................0.1g Thiamine .........................................................1.0mg Vitamin B12 ......................................................1.0µg Glucose solution .......................................... 10.0mL Vitamin solution........................................... 10.0mL Trace elements solution ................................. 1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution: Composition per 100.0mL: Glucose ............................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
