ABSTRACT

The main goal of array image processing is to measure the intensity of the spots and quantify the gene expression values based on these intensities. A very important and often neglected goal is also assessing the reliability of the data, and generating warnings signaling possible problems during the array production and/or hybridization phases. This chapter is divided into two parts. Section 5.2 provides a very short description of the basic notions involved in digital imaging. Section 5.3 and following focus on image processing issues specific to microarrays.