chapter  6
26 Pages

In Vitro Models for Studying Induction of Cytochrome P450 Enzymes

Isolation of viable hepatocytes was first demonstrated by Howard et al. and rapidly increased in popularity with the further development of a high-yield preparative technique by Berry and Friend (6,7). Compared with liver slices, isolated hepatocytes are easier to manipulate and show a superior range of activities (8). For a detailed description of rat and human hepatocyte isolation techniques, the reader is referred to other reviews (8,9).