In Vitro Models for Studying Induction of Cytochrome P450 Enzymes
Isolation of viable hepatocytes was first demonstrated by Howard et al. and rapidly increased in popularity with the further development of a high-yield preparative technique by Berry and Friend (6,7). Compared with liver slices, isolated hepatocytes are easier to manipulate and show a superior range of activities (8). For a detailed description of rat and human hepatocyte isolation techniques, the reader is referred to other reviews (8,9).