ABSTRACT
Robinia pseudoacacia is a leguminous tree species that has been planted worldwide. In Japan, it is used mainly for landscaping and production of honey. Much research related to in vitro regeneration systems of R. pseudoacacia has been reported (Han, Keathley, and Gordon, 1993). However, to our knowledge, only one example of Agrobacterium rhizogenes-mediated transformation of R. pseudoacacia has been documented (Han, Keathley, Davis et al., 1993). The reported transformation resulted in abnormal leaf morphology. We established a new, simple, and reliable procedure for the transformation of R. pseudoacacia by disarmed A. tumefaciens (Igasaki et al., 2000). The leaves of transgenic plants generated by our method exhibited no apparent morphological abnormalities.
