ABSTRACT
Development of plant tissue culture and plant cell transformation techniques has revolutionized plant biology. Although plant transformation is technically less demanding and is now within the reach of any modest laboratory, reproducible and efficient protocols in a number of legumes are yet to be established. In all such cases where transformation protocols are established, researchers from a variety of disciplines can employ this approach to find answers to specific questions. At the outset, the cause and effect relations in transformation experiments appear straightforward. However, in practice, all effects observed are not directly attributable to the transgene introduced through transformation. While a large body of literature is available on transformation of various plant species, discussion on genetic and molecular analyses of transgenic plants is limited. Since the methods of analysis are not specific to legumes, in this chapter we discuss the appropriate methods and aspects that need to be considered while analyzing the results of transformation experiments.
