ABSTRACT
Themolecular recognition abilities ofMIPs are clearly theirmost importantmaterial property.The molecular a⁄nity and selectivity form the basis for the utility of MIPs in chromatography [1], biosensors [2], and catalysis applications [3]. Therefore, the ability to accurately quantify and measure these properties is an essential tool for the veri¢cation of the imprinting process and for the optimization ofMIPs.The binding properties ofMIPs can bemeasured in chromatographic, solid-phase extraction, and membrane transport studies. In each of these analyses, binding properties can be calculated from the corresponding experimental binding isotherms. The extraction of binding properties from isotherms requires the application of speci¢c binding models and assumptions about the distribution of binding sites inMIPs.This chapter will review the use of binding isotherms to characterize MIPs and, in particular, review practical aspects of applying di¡erent binding models to MIPs. Other chapters in this volume may also be referred with respect to binding analyses; batch rebinding (Chapters16, 22), chromatography (Chapter 20).
