ABSTRACT
Immunoproteomics........................................................................... 413 20.3.2 Chapter 10: Near-Infrared Fluorescence Detection of
Antigen-Antibody Interactions on Microarrays.............................. 413 20.3.3 Chapter 11: Application of Shotgun Proteomics to
Transcriptional Regulatory Pathways .............................................. 414 20.3.4 Chapter 12: Electrophoretic NMR of Protein Mixtures and
its Proteomics Applications ............................................................. 414
20.4 Part IV. Chemical Proteomics: Studies of Protein-Ligand Interactions in Pools and Pathways ..................................................................................... 415 20.4.1 Chapter 13: Characterizing Proteins and Proteomes Using
Isotope-Coded Mass Spectrometry.................................................. 415 20.4.2 Chapter 14: Surface Plasmon Resonance Biosensors’
Contributions to Proteome Mapping ............................................... 415 20.4.3 Chapter 15: Application of In-Cell NMR Spectroscopy to
Investigation of Protein Behavior and Ligand-Protein Interaction inside Living Cells ........................................................................... 416
20.4.4 Chapter 16: An Overview of Metabonomics Techniques and Applications ..................................................................................... 416
20.5 Part V: Structural Proteomics: Parallel Studies of Proteins ........................ 417 20.5.1 Chapter 17: NMR-Based Structural Proteomics ............................. 417 20.5.2 Chapter 18: Leveraging X-Ray Structural Information in Gene
Family-Based Drug Discovery: Application to Protein Kinases .... 418 20.5.3 Chapter 19: EPR Spectroscopy in Genome-Wide
Expression Studies ........................................................................... 418
Proteomic studies strive to characterize groups of proteins in a systematic manner. This can involve studies of pools of proteins isolated from tissue extracts or subsets (subproteomes) of these protein mixtures (part II). Studies can also be of individual purified proteins, but carried out in a highly parallel way, as in structural proteomics. In both cases, studies are “systems based” (chapter 1) because they focus on groups of proteins that are related by the networks of interactions they participate in or by similarities in their binding sites.
