ABSTRACT
Fluorescence is the phenomenon in which absorption of light at a given wavelength by a fluorescent molecule is followed by the emission of light at longer wavelengths. Some of the advantages of fluorescence regarding screening assay development are its nonradioactive nature, its high sensitivity, and its safety. Safety refers to the fact that it is a noninvasive technique that does not affect
or destroy samples in the process. The fluorescence signal is proportional to the concentration of the substance being investigated, and techniques using fluorescence can accurately detect concentrations in the pico-and even femtomolar range.
