ABSTRACT
I. Introduction ..................................................................................................495 A. Incorporation of Synthetic Precursors into the Bacterial Cell Wall ........498 B. Adhesion of Surface-Engineered Bacteria to the
Con A-immobilized Film by Displaying Carbohydrates ......................500 C. Modification of Cell-Wall Precursors ..................................................501 D. Enhancement of Incorporation Efficiency by Antibiotics ....................503
II. Conclusions ..................................................................................................504 References ..............................................................................................................505
G. P. Smith’s report on the display of target proteins on phage surfaces,1 a technique now generally referred to as phage display, was the precursor of many later studies that sought to apply the same technique to the display of large proteins on microbial cells. Subsequently, target proteins fused with a cellular anchor protein have been successfully displayed on the cell surface of Escherichia coli and yeast.2,3 This strategy is based on a genetic method using recombinant DNA corresponding to the target and anchor proteins.4,5
