chapter  11
28 Pages

Protein Expression Analysis

The technologies currently available that allow the quantification of protein expression in clinical samples include two-dimensional polyacrylamide gel electrophoresis (2D PAGE), multidimensional chromatography, protein arrays, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), surface-enhanced laser desorption/ionization (SELDI), and tissue arrays.1,2 2D PAGE has been the mainstay of electrophoretic technologies and is the most widely used tool for protein separation and quantification. In this chapter, as a practical example of the use of expression proteomics, we will describe the methods utilized for the analysis of the quantitative expression of a large number of proteins in a relatively large number of individual lung tissue samples using 2D PAGE.