chapter  6
14 Pages

Carbonic Anhydrase-Based Biosensing of Metal Ions: Issues and Future Prospects

Over the past decade, fluorescence-based determinations of free metal ions in solution using variants of human apocarbonic anhydrase (apoCA) have been demonstrated to be highly sensitive, selective, and versatile. In particular, free divalent copper, zinc, cobalt, cadmium, and nickel have been determined at concentrations down to the picomolar range

by changes in fluorescence emission

and excitation wavelength ratios,

lifetimes,

and anisotropy (polarization).