chapter  2
40 Pages

Prospects for the De Novo Design of Nucleic Acid Biosensors

Selection of Functional Nucleic Acids...............................................6 2.3 Nucleic Acid Biosensors..................................................................................7

2.3.1 Fluorescent Signals Resulting from Analyte Capture .........................7 2.3.2 Fluorescent Signals Resulting from Conformational

Transduction .........................................................................................8 2.3.2.1 Small Motions.......................................................................8 2.3.2.2 Large Motions.....................................................................11 2.3.2.3 Global Structural Rearrangements .....................................12 2.3.2.4 Quaternary Structural Rearrangements ..............................17 2.3.2.5 Aptazymes...........................................................................17 2.3.2.6 Aptazyme Assays................................................................22

2.4 Prospects for the Design of Nucleic Acid Biosensors ..................................27 2.4.1 Mechanisms for Oligonucleotide Dependence..................................27