ABSTRACT
The above quote was written when polymerase chain reaction (PCR)-based molecular methods have not yet arrived at the fungal taxonomic scene and especially for mycologists’ studying dermatophytes. The PCR technology as we know now was only available when Kary B. Mullis made a breakthrough of amplifying DNA in vitro,2 and 2 years later the use of thermostable polymerase3 made the whole process automated, which revolutionized biological investigations. This single method has undoubtedly had more application in biology today than any other technique in whole of biological sciences including diagnosing human fungal infections.
