ABSTRACT
The application of nucleic acids as therapeutic agents in antisense and triplex technologies — and as molecular biology tools in the field of diagnostics — inspired researchers to study various nucleobase, sugar, and phosphate backbone modifications of the naturally occurring nucleic acids. As a result, several new inventions largely responsible for the successful sequencing of the human genome, such as polymerase chain reaction (PCR) and DNA sequencing technologies, were developed. They are also widely used techniques in molecular diagnostics. BM-NTPs were critical components of the success of this endeavor. In addition, a tremendous amount of work published in the last three decades has improved understanding of nucleic acids with various chemical modifications.
