ABSTRACT

Imaging of chemical and biological systems based on various spectroscopic techniques is a subject of wide interest. In fact, microscope imaging modes can be based on a wide range of physical principles. There are several types of microscopes: conventional (nonscanning), scanning, and confocal. In fact, microscope imaging modes can be based on any spectroscopy that measures a spatial variation in the signal. Throughout this book, we have seen nonlinear optical processes that are coherent, such as second-harmonic generation; and incoherent, such as hyper-Rayleigh scattering. Hence, the same classification occurs when coupling any of these techniques with a microscope.