ABSTRACT
The advent of high throughput technologies over the last two decades (e.g., DNA sequencing, gene microarrays, etc) have led to fundamental insights into the workings of various biological processes. In addition they have provided inferences about protein interactions and regulation. However, they have limitations compared to the direct observations of protein expression levels. High throughput technologies that facilitate this task would enable researchers to build more accurate and comprehensive protein pathways. However, until recently most methods used for inferring protein expression levels, such as Western blots, 2D gel electrophoresis and more recently antibody protein microarrays, are inherently low-throughput.
