Chapter Recombinant Food Allergens and Their Role in Immunoassay and Immunotherapy
In the recent years, standardization and characterization of allergen extracts from natural source have been improved, but extracts are often heterogeneous and may contain a mixture of nonallergenic molecules and relevant allergens which can vary in composition and quantity (Chapman et al. 2002). Molecular biology could be the solution for these problems. Many recombinant food allergens have been expressed using various systems, both prokaryotic and eukaryotic. The large-scale production of such de ned molecules facilitates the proper molecular and immunological characterization of allergens, and offers new perspectives in allergy diagnosis, research, and therapy.