ABSTRACT
CONTENTS 3.1 Introduction .............................................................................................................33 3.2 Conventional Methods for Peptide Analysis ........................................................... 34
3.2.1 Electrophoretic Methods ............................................................................. 34 3.2.2 Chromatographic Methods ......................................................................... 40 3.2.3 Spectroscopic Techniques .............................................................................45 3.2.4 Immunochemical Methods ......................................................................... 46
3.3 Mass Spectrometry for Peptide Analysis...................................................................47 3.3.1 Instrumentation ...........................................................................................47 3.3.2 Tandem Mass Spectrometry ........................................................................ 49 3.3.3 Interpretation of Mass Spectra .................................................................... 49 3.3.4 Applications to Dairy Peptides .................................................................... 50
3.3.4.1 Milk Proteomics ........................................................................... 50 3.3.4.2 Biologically Active Peptides ...........................................................51 3.3.4.3 Evaluation of Proteolysis................................................................59 3.3.4.4 Quantitative Analysis of Peptides by Mass Spectrometry ..............59
