ABSTRACT
Contents 6.1 Introduction ..................................................................................................................... 70
6.1.1 General Aspects of Lipid Compounds .................................................................. 70 6.1.2 Marine Lipid Characteristics ................................................................................ 70 6.1.3 Lipid Analysis in Marine Products ........................................................................ 71
6.2 First Steps in Marine Lipid Analysis ................................................................................. 72 6.2.1 Isolation of Lipids from Tissues ............................................................................ 72 6.2.2 Removal of Nonlipid Contaminants ..................................................................... 72 6.2.3 Lipid Manipulation and Storage ........................................................................... 73 6.2.4 Lipid Quanti cation ............................................................................................. 73
6.3 Marine Fatty Acid Analysis .............................................................................................. 73 6.3.1 Fatty Acid Methyl Esters Preparation ................................................................... 73
6.3.1.1 Acid-Catalyzed Esteri cation and Transesteri cation ............................. 73 6.3.1.2 Base-Catalyzed Transesteri cation ......................................................... 75
6.3.2 GLC Analysis of FAME ........................................................................................ 75 6.3.2.1 Qualitative Analysis of Fatty Acid Composition ..................................... 75 6.3.2.2 Quantitative Estimation of Fatty Acid Composition ...............................76
6.4 Analysis of Marine Nonsaponi able Matter ......................................................................76 6.4.1 Lipid Saponi cation ...............................................................................................76 6.4.2 Analysis of Sterols ..................................................................................................76 6.4.3 Analysis of Ether Lipids ........................................................................................ 77
6.5 Qualitative and Quantitative Analyses of Marine Lipid Classes ....................................... 77 6.5.1 Spectrophotometric Assessments of Total Lipid Extract ........................................ 78 6.5.2 Stereospeci c Analysis of Lipid Classes ................................................................. 78 6.5.3 Column Chromatography..................................................................................... 79
