chapter  1
Optical Imaging of Brain Activity In Vivo Using Genetically Encoded Probes
Pages 36

Expression ........................................................................................... 10 1.3.5 Recombinase-Based Systems for Cell Type-Specic Targeting ........ 11

1.4 Experimental Setup for Imaging with Genetically Encoded Reporters .......... 11 1.4.1 Optical Setup ...................................................................................... 11 1.4.2 Physical Setup for In Vivo Imaging .................................................... 13 1.4.3 Repeated, Chronic In Vivo Imaging.................................................... 14 1.4.4 Imaging in Awake, Head-Fixed Animals ............................................ 15

1.5 Signal Correction and Analysis Methods ........................................................ 16 1.5.1 Photobleaching ................................................................................... 16 1.5.2 Hemodynamic Artifacts ...................................................................... 17 1.5.3 Green Autouorescence Signals ......................................................... 19 1.5.4 Movement Correction ......................................................................... 21 1.5.5 Estimating Action Potential Firing from Optical Signals ................... 21

1.6 Example Experiments .....................................................................................22 1.6.1 SpH Imaging of Odorant-Evoked Activity and Presynaptic

Modulation of Transmitter Release ....................................................22 1.6.1.1 SpH as a Reporter of Transmitter Release ...........................23 1.6.1.2 Using SpH to Probe the Organization of Presynaptic

Inhibition In Vivo .................................................................27 1.6.2 Imaging Postsynaptic Odor Representations with GCaMP2 ..............29