chapter  4
MPScope 2.0: A Computer System for Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation and Electrophysiology
Pages 26

Acknowledgments .................................................................................................. 137 Appendix ................................................................................................................ 138 References .............................................................................................................. 141

The MPScope1 freeware package is a comprehensive suite of programs that run under the Microsoft Windows operating system and are designed as control and analysis elements for two-photon laser scanning microscopy (TPLSM) and various extensions. MPScope consists of MPScan, the acquisition software, MPView, the analysis program, and MPFile, a utility to open MPScope data les in user-written applications. MPScope was designed from the outset as a turn-key, stand-alone, easy-to-use software system with exible hardware options aimed at concurrent functional imaging, (e.g., calcium uorescence), and electrophysiology experiments. However, the versatility of MPScope is such that it can be used in many different types of experiments including in vivo cortical blood ow imaging and two-photon plasma-mediated ablation automated histology.2 The usefulness of MPScope stems mostly from leveraging advanced software technologies available on the Windows platform.