ABSTRACT
During the past decade, Agrobacterium-mediated genetic transformation became one of the dominant technologies used to produce a variety of genetically modied transgenic plants. The successful introduction of this technology relies on the fact that Agrobacterium tumefaciens is a typical plant pathogen in soil. However, in contrast to many other plant pathogens, it has a unique ability of trans-kingdom DNA transfer (reviewed in Gelvin 2003). The wide popularity of this biotechnology tool is reected by the growing number of Agrobacterium-related patents published yearly (Roa-Rodriguez and Nottenburg 2003). A broad range of host species from plant to
7.1 Introduction ....................................................................................................99 7.2 Experimental System Allowing Rapid Identication of Chemicals That
Inuence HRF .............................................................................................. 100 7.3 Strategies for the Development of a Growth Medium Enhancing HRF....... 101
7.3.1 Analysis of HRF in the MS Medium Depleted of Certain Chemicals ......................................................................................... 101
7.3.2 Analysis of HRF in the Medium Enriched with Various Compounds ....................................................................................... 103
7.3.3 The Inuence of Combinations of Various Chemicals on HRF ....... 105 7.3.4 Effects of Growth Medium Enriched with G Chemical on Stable
Transformation Frequency ................................................................ 106 7.4 Materials and Methods ................................................................................. 107
7.4.1 Description of a Transgenic Line Used for Experiments ................. 107 7.4.2 Calculation of HRF and RR ............................................................. 107 7.4.3 Growth Media Genotoxicity Assessment ......................................... 109 7.4.4 Plant Transformation ........................................................................ 109
7.5 Conclusions ................................................................................................... 109 Acknowledgments .................................................................................................. 110 References .............................................................................................................. 110
yeast and even to human cells (Tzra and Citovsky 2003, reviewed in Lacroix et al. 2006) has been shown to be transformed with Agrobacterium.
