ABSTRACT
Tolerant Deoxyriboaldolase from Klebsiella pneumoniae ........................... 271 19.3 Efcient Production of DR5P from Glucose and Acetaldehyde via FDP
by Coupling of Alcoholic Fermentation System of Baker’s Yeast and Deoxyriboaldolase-Expressing E. coli ......................................................... 271
19.4 Biochemical Retrosynthesis of 2′-Deoxyribonucleosides from Glucose, Acetaldehyde, and a Nucleobase .................................................................. 273
19.5 Improvement of the One-Pot Multistep Enzymatic Process for Practical Production of 2′-Deoxyribonucleoside from Glucose, Acetaldehyde, and a Nucleobase ................................................................................................. 275
19.6 Conclusions ................................................................................................... 275 Acknowledgments .................................................................................................. 277 References .............................................................................................................. 277
There will be a need for 2′-deoxyribonucleoside in the near future due to increasing demand in new medical and biotechnology elds. 2′-Deoxyribonucleoside is a building block of promising antisense drugs for cancer therapy. For some recently developed antiviral reagents, such as azidothymidine for treatment of human immunodeciency virus (HIV) infections, 2′-deoxyribonucleoside is a synthesis intermediate. 2′-Deoxyribonucleoside is also a precursor of an indispensable material used for widespread polymerase chain reaction (PCR) applications, 2′-deoxyribonucleoside triphosphate. The current 2′-deoxyribonucleoside sources include hydrolyzed herring and salmon sperm DNA, which are not suitable sources for sudden high demands. Microbial/enzymatic processes for 2′-deoxyribonucleoside production could possibly remove this bottleneck of raw material supply [1].
