ABSTRACT
For more than 20 years, various industrial, governmental, and academic laboratories have developed and rened genetic engineering strategies aimed at manipulating lipid metabolism in plants and microbes. The goal of these projects is to produce
2.1 Introduction .................................................................................................... 19 2.2 Castor and Tung: Textbook Examples of the Advantages and
Disadvantages of Exotic Oilseed Species ....................................................... 21 2.3 Primary Biosynthetic Enzymes Are Insufcient for High-Level
Production of Unusual Fatty Acids .................................................................22 2.4 Various Enzymes of the Kennedy Pathway and Land’s Cycle May Be
Necessary for Efcient Packaging of Unusual Fatty Acids in Storage Lipids ..............................................................................................................23 2.4.1 Diacylglycerol Acyltransferase ...........................................................23 2.4.2 GPAT, LPAT, LPCAT, PDAT, and CPT: An Alphabet Soup of
Enzymes That May Hold the Keys to Ultimate Success ....................25 2.4.2.1 The Other Kennedy Pathway Acyltransferases ...................25 2.4.2.2 The Land’s Cycle Enzymes and Other Acyltransferases .....27