ABSTRACT
Supercritical uid chromatography (SFC) has long held an uncertain place among other separation techniques. However, recently, packed column SFC (pSFC) has become more attractive again to drug discovery [1-4] because it offers aqueous-free purication capabilities with a reputed green solvent (carbon dioxide), together with
5.1 Introduction .................................................................................................. 195 5.2 Experimental Conditions .............................................................................. 201
5.2.1 Chromatographic System ................................................................. 201 5.2.2 Choice of Chromatographic Conditions ...........................................202 5.2.3 Selection of Columns ........................................................................203 5.2.4. Selection of a Set of Test Compounds ..............................................206 5.2.5 Data Analysis .................................................................................... 218
5.3 Choice of Solvation Descriptors for Supercritical Fluids .............................225 5.4 A General Database for Packed Column SFC ..............................................228
