ABSTRACT
Agarose is a well-established material in many applications of biochemistry and biotechnology. Agarose gels are used for electrophoresis of very large molecules such as DNA and agarose-based particles are used as chromatographic media for separation of all sorts of biomolecules, particularly
CONTENTS
7.1 Introduction ................................................................................................ 155 7.2 Preparation of Superporous Gels ............................................................ 157
7.2.1 Superporous Particles ................................................................... 157 7.2.2 Superporous Monoliths ................................................................ 158 7.2.3 Superporous Composite Gels ....................................................... 160
7.3 Characterization of Superporous Gels ................................................... 161 7.3.1 Microscopy Characterization ....................................................... 161 7.3.2 Measuring Pore Flow .................................................................... 162 7.3.3 Chromatographic Characterization Height Equivalent
to a Theoretical Plate (HETP) ....................................................... 165 7.4 Derivatization of Superporous Gels ........................................................ 166 7.5 Applications of Superporous Agarose .................................................... 166
7.5.1 Superporous Agarose for Protein Separation ............................ 168 7.5.2 Superporous Agarose for Plasmid Isolation .............................. 170 7.5.3 Enzyme Reactors ............................................................................ 172 7.5.4 Superporous Agarose Applications in Bioanalysis
and Biosensors ................................................................................ 172 7.5.5 Superporous Agarose Employed as Internally Cooled
Electrophoresis Gels ...................................................................... 175 7.6 Concluding Remarks ................................................................................. 176 Acknowledgment ................................................................................................ 176 References ............................................................................................................. 177
proteins. Prominent commercial examples of agarose-based separation particles are exemplied by the brand names Sepharose, Uniow, Af-gel, and Superose. The chromatography particles are available for many modes of chromatography, for example, gel ltration, ion exchange chromatography, hydrophobic interaction chromatography, and afnity chromatography.
