ABSTRACT
Protein interactions are an important layer of connectivity between cell components and cell processes. They allow the formation of protein complexes and mediate post-translational protein modifications. Disruption of protein-protein interactions may result in disruption of the cell component or process to which they contribute, compromising the cell viability or even leading to cell death. The study of protein interactions and their function has recently jumped to an organism scale, following the development of high-throughput yeast-two-hybrid screens mapping protein-protein interactions (1-7) and affinity purification followed by mass spectrometry (MS) detecting protein complexes (8,9) (see Chapters 5, 6, and 7 for a thorough discussion of mass spectrometry and MS-based tissue imaging).
