chapter  11
18 Pages

Neuroproteomics in the Neocortex of Mammals: Molecular Fingerprints of Cortical Plasticity

With the accumulation of vast amounts of DNA sequences in databases, researchers are realizing more and more that having complete sequences of genomes is not sufficient to elucidate biological function, not even if complemented with detailed information on the dynamics of the transcriptome. A cell or tissue is dependent on a huge number of metabolic and regulatory pathways for its normal functioning and there is no strict linear relationship between gene expressions and the protein complements or “proteome.” Proteomics is therefore complementary to genomics and transcriptomics because it focuses on the gene products, the true active agents in a cell or tissue at any given time during a given physiological state. The advent of proteomics techniques has been enthusiastically accepted in most areas of biology and medicine. In neuroscience a host of applications was proposed ranging from neurotoxicology, neurometabolism, and determination of the proteomes of individual brain regions in health and disease, to name a few. We have implemented functional proteomics to help unravel the molecular pathways of brain plasticity that drive the response of

11.1 Introduction .................................................................................................. 197 11.2 Facts about Cat Visual System ..................................................................... 198 11.3 Neuroproteomics of Cat Visual Cortex Plasticity ........................................ 199 11.4 CRMPs and Cortical Plasticity ..................................................................... 201 11.5 Dynamin I and Mature Cell Shape ...............................................................207 11.6 Synaptotagmin I and Cortical Plasticity .......................................................208 11.7 Conclusion ....................................................................................................209 11.8 Future Challenges .........................................................................................209 Acknowledgments ..................................................................................................209 References .............................................................................................................. 210

the mammalian sensory neocortex to stimulus deprivation during development and adulthood, with a special emphasis on the visual system of the cat.