High-Density Genomic Tiling Arrays
A large-scale international project by the ENCODE consortium studying the regulatory elements in 1 percent of human genome revealed significant roles and activities of histone modifications in gene transcription and DNA replication. One of the major technologies in the endeavor was tiling arrays, which consist of oligonucleotide probes from the entire ENCODE region, including introns within genes and regions between genes (but excluding repetitive sequences). The pilot project also assessed and developed protocols of highdensity tiling arrays and found the hybridization results were reproducible across platforms and laboratories. As a trend is seen toward tiling array assay, we dedicate this chapter to the analysis.