chapter  6
24 Pages

Chapter Chiral Separations Using Proteins and Peptides as Chiral Selectors

Drug enantiomers can be different in potency, toxicity, and behavior in biological systems [1]. Thus, a lot of analytical methods have been developed for the discrimination of drug enantiomers. Among those, chiral high-performance liquid chromatography (HPLC) methods are widely employed for the assays of drug enantiomers in pharmaceutical preparations and biological uids [2-4]. Recently, capillary electrophoresis (CE) methods have been developed for the separation of drug enantiomers by using chiral selectors, such as chiral ligand exchange, cyclodextrins, crown ethers, chiral micelles, polysaccharides, proteins, peptides, macrocyclic antibiotics, and molecularly imprinted polymers, as the running buffer additives or

immobilized ligands [3]. Among those, proteins and peptides are of special interest as chiral selectors because of their unique properties of stereoselectivity and/or their suitability for separating a wide range of enantiomeric mixtures [3,5,6].