ABSTRACT
Advances in recombinant DNA technology, genetics, and biotechnology have enabled the biosynthesis and production of complex therapeutic proteins in a variety of systems. Once they have been synthesized intracellularly in the host organism, these proteins are either
exported into the culture medium or retained intracellularly as inclusion bodies. The purification method that is used for these proteins will depend on their concentrations and their final state in the raw sample. This chapter examines the use of antibodies and other affinity ligands for the purification of recombinant proteins from complex mixtures.
