ABSTRACT
Expression for a Multivalent Solute..................................................... 640 23.4.2 Linearity of the Conventional Scatchard Plot
for a Multivalent Solute........................................................................ 641 23.4.3 The Rectangular Hyperbolic Relationship
for a Multivalent Partitioning Solute.................................................... 642 23.5 Biomembrane-Affinity Chromatography ............................................................ 643
23.5.1 Studies with Erythrocyte Membranes as the Affinity Matrix.............. 643 23.5.2 Erythrocyte Ghosts as an Affinity Matrix ............................................ 645
23.6 Evaluation of Rate Constants by Affinity Chromatography............................... 646 23.6.1 Theoretical Considerations ................................................................... 646 23.6.2 Experimental Applications.................................................................... 647
23.7 Impact of Biosensor Technology ........................................................................ 648
23.7.1 Biosensors in Thermodynamic Studies ................................................ 649 23.7.1.1 Interaction between Interleukin-6 and a Soluble
Form of Its Biospecific Receptor ....................................... 649 23.7.1.2 Allowance for Solute Depletion in Experiments
with Cuvette-Based Biosensors .......................................... 649 23.7.1.3 Contribution of Biosensor Studies
to an Understanding of RNA Trafficking........................... 650 23.7.2 Kinetic Analysis of Biosensor Data ..................................................... 652
23.7.2.1 Pseudo-First-Order Kinetics................................................ 652 23.7.2.2 Second-Order Kinetics ........................................................ 653 23.7.2.3 Biacore Analysis of a 1:1 Interaction ................................. 654
23.8 Summary and Conclusions.................................................................................. 655 Symbols and Abbreviations ............................................................................................ 656 References ....................................................................................................................... 657
23.1 INTRODUCTION
Quantitative affinity chromatography refers to the characterization of biospecific interactions by methods that are essentially adaptations of preparative affinity techniques. This use of affinity chromatography was given extensive coverage in the previous edition of this handbook [1], which remains a valid summary of the first 20 years in this field. The basic principles behind quantitative affinity chromatography are described here in Chapter 22 of this current book, with the main emphasis in this chapter being recent theoretical developments that have occurred in this area.
