ABSTRACT
A key factor in the success of any type of affinity chromatography is the way in which the ligand is attached to its support [1-3]. Ideally, the chosen ligand should mimic its interactions within its natural environment. Thus, it is important to consider the way in which the ligand is placed within the column. The success of this process depends on the selection of an appropriate immobilization method. This chapter examines several immobilization methods for affinity chromatography and discusses the relative advantages and disadvantages of each.
