ABSTRACT
Although affinity chromatography can employ a variety of ligands, most of these tend to be of biological origin. The use of a biological ligand within a column is sometimes known as bioaffinity chromatography or biospecific adsorption. This was the first type of affinity chromatography developed, as used by Starkenstein in 1910 when he purified α-amylase using insoluble starch [1]. The specificity of most biological ligands makes bioaffinity chromatography an ideal technique for the purification of numerous compounds. This method has also become important as a tool in the analysis and characterization of biological samples.
