ABSTRACT
Contents Introduction ............................................................................................................ 128 List of Protocols ..................................................................................................... 129 Protocol 8.1: Design and Construction of Sugar Arrays ........................................ 130 Protocol 8.2: Micro Spotting of Carbohydrates onto Bioarray
Substrates ........................................................................................................... 131 How It Works ................................................................................................ 131 Required Materials ........................................................................................ 131
Apparatus .......................................................................................... 131 Softwares .......................................................................................... 131 Antibodies ......................................................................................... 131 Reagents and Buffers ........................................................................ 132
Protocol 8.2.1: Printing of Carbohydrate Arrays onto Nitrocellulose Slides ................................................................................. 132
Step-by-Step Protocol ....................................................................... 132 Protocol 8.2.2: Preparation of Photo-Generated Glycan Arrays on
PAM Slides ............................................................................................... 132 Step-by-Step Protocol ....................................................................... 132
Protocol 8.3: Immunostaining of Carbohydrate Microarrays ................................ 133 Protocol 8.4: Microarray Scanning and Data Collection ....................................... 133 Protocol 8.5: Microarray Data Processing and Standardization ............................ 133 Protocol 8.6: Validation and Further Investigation of Microarray
Observations....................................................................................................... 134 Protocol 8.7: Probing Immunogenic Sugar Moieties Using
Sugar Arrays ....................................................................................................... 134 Acknowledgment ................................................................................................... 136 Key References ...................................................................................................... 136 Troubleshooting Guide ..........................................................................................136
