ABSTRACT

Contents Introduction .............................................................................................................. 15

Extraction ........................................................................................................ 15 Purification ...................................................................................................... 16 Electrophoretic Analysis ................................................................................. 16 Labeling Strategies ......................................................................................... 17 Storage ............................................................................................................ 17

List of Protocols ....................................................................................................... 18 Protocol 2.1: Tissue Homogenization ...................................................................... 18

How It Works .................................................................................................. 18 Required Materials .......................................................................................... 19

Homogenizer Types: Select type based upon tissue and preparation scale ............................................................................. 19

Other Materials ...................................................................................20 Reagents and Buffers ..........................................................................20