ABSTRACT
INTRODUCTION The study of nanoparticle cell integration involves various techniques based on the nature of the nanoparticle and the cells involved. Some general methods and instrumentation used for cytomic study are discussed in this chapter. Flow cytometry uses the principles of light scattering, light excitation, and emission of fluorochrome molecules to generate specific multiparameter data from particles and cells in the size range of 0.5 to 40 m. Cells are hydrodynamically focused in a sheath of phosphate-buffered saline (PBS) before intercepting an optimally focused light source (1). Lasers are most often used as a light source in flow cytometry.
