ABSTRACT
Activity......................................................................................................... 195 9.4 New Possibilities and Approaches to Biological Detection
of Plant Substances ...................................................................................... 196 9.4.1 Separation Techniques ..................................................................... 196
9.4.1.1 Conventional Planar Layer Liquid Chromatographic Techniques........................................................................ 196
9.4.1.2 Forced Flow Planar Layer Liquid Chromatographic Techniques........................................................................ 196
9.4.2 Biodetection ..................................................................................... 197 9.4.3 Spectroscopic (MS, FT-IR, NMR, etc.) Evaluation
of Chromatographic Spots before and after Inoculation ................. 197 9.4.4 Optimization of Parameters in Direct Bioautography
and Some of Their Technical Solutions .......................................... 198 9.5 Interaction Guided Separation and Detection of Plant Substances
in the Adsorbent Layer-BioArena System................................................ 199 9.5.1 Basic Elements of BioArena System and Its Importance ............... 199 9.5.2 Occurrence and Role of HCHO in Biological
Systems-Formaldehydome System ............................................... 200 9.5.3 Interactions between HCHO and H2O2........................................... 201 9.5.4 Effect of Deprivation and Mobilization of HCHO
on the Antibacterial Activity ........................................................... 202 9.5.4.1 Interaction and Double Effect
of Trans-Resveratrol ......................................................... 202
9.5.4.2 Study of ‘‘Classical Medicines’’ in BioArena System ..... 203 9.5.5 Elimination of O3 from the Chromatographic Spots
and Its Effect on the Antibacterial Activity of Antibiotics ............. 206 9.5.6 Time-Dependent Change of Spot Color
of Trans-Resveratrol (TR) in BioArena as a Manifestation of Biochemical Changes .................................................................. 207
9.5.7 BioArena as an Alternative Animal Experimental Model .............. 207 9.6 Bioassay-Guided Separation and Isolation of Biologically Active
Plant Substances........................................................................................... 208 9.7 Conclusions.................................................................................................. 209 References ............................................................................................................. 209
There are three main mechanisms by which one microorganism may limit the growth of another microorganism: antibiosis, micro-parasitism, and competition for resources [1]. Viullemin in l899 applied the word antibiosis to situations in which one organism destroyed another to sustain its own life [2]. Among the cell proliferation promoting and retarding factors, antibiotics are the most important molecules originating from the antibiosis processes. The word antibiotic is very broad and includes widely disparate mechanisms. Microbial toxins have recently been discussed in detail [3]; they are usually considered to be poisonous to higher animals and humans. The antibiotic penicillin can be a toxin to a person sensitive to it.
