ABSTRACT
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Chromatographic techniques are useful analytical methods for separation, identication, and quantitative determination of a wide variety of compounds from a wide variety of matrices. Many components of interest have been identied in phytochemical analysis (proteins, carbohydrates, lipids, alkaloids, phenolics, terpenoids, and so on), and their quantication with high performance liquid chromatography (HPLC) always follows the same rules [1]. Chromatography is primarily a comparative analytical technique. The values generated by the detector are not directly usable and must be changed in concentrations. Thus, chromatography involves a calibration that is the key operation in a chemical measurement process.
